Drosha processing controls the specificity and efficiency of global microRNA expression
نویسندگان
چکیده
منابع مشابه
Drosha in primary microRNA processing.
MicroRNA (miRNA)-mediated gene silencing is one of the major regulatory pathways in eukaryotes. Much effort has been made to identify the factors involved in the pathway, and our understanding of RNA silencing has significantly advanced in recent years. Our group has been working on some of the issues regarding miRNA biogenesis and, in this paper, we summarize what we and other workers in the f...
متن کاملThe terminal loop region controls microRNA processing by Drosha and Dicer
microRNAs are widely expressed, ∼22-nt-long regulatory RNAs. They are first transcribed as much longer primary transcripts, which then undergo a series of processing steps to yield the single-stranded, mature microRNAs, although the mechanisms are incompletely understood. Here, we show that the terminal loop region of human primary microRNA transcripts is an important determinant of microRNA bi...
متن کاملThe Drosha-DGCR8 complex in primary microRNA processing.
RNase III proteins play key roles in microRNA (miRNA) biogenesis. The nuclear RNase III Drosha cleaves primary miRNAs (pri-miRNAs) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic RNase III Dicer to generate mature miRNAs. While Dicer (class III) and other simple RNase III proteins (class I) have been studied intensively, the class II enzyme Drosha remains to be...
متن کاملReliable prediction of Drosha processing sites improves microRNA gene prediction
MOTIVATION Mature microRNAs (miRNAs) are processed from long hairpin transcripts. Even though it is only the first of several steps, the initial Drosha processing defines the mature product and is characteristic for all miRNA genes. Methods that can separate between true and false processing sites are therefore essential to miRNA gene discovery. RESULTS We present a classifier that predicts 5...
متن کاملOncogenic All1 fusion proteins target Drosha-mediated microRNA processing.
MicroRNAs (miRNAs) are noncoding small RNA of approximately 22 bases, which suppress expression of target genes through translational block or degradation of a target's transcript. Recent studies uncovered specific miRNA expression profiles in human malignancies. Nevertheless, the mechanisms underlying cancer-specific miRNA expression are largely unknown. miRNA biogenesis consists of a series o...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
سال: 2011
ISSN: 1874-9399
DOI: 10.1016/j.bbagrm.2011.05.015